Microfluidic cell sorter sample preparation for genomic assays

Single-cell RNA-Sequencing has led to many novel discoveries such as the detection of rare cell populations, microbial populations, and cancer mutations. The quality of single-cell transcriptomics relies heavily on sample preparation and cell sorting techniques that best preserve RNA quality while removing dead cells or debris prior to cDNA generation and library preparation. Magnetic bead cell enrichment is a simple process of cleaning up a sample but can only separate on a single-criterion. Droplet-based cell sorters, on the other hand, allows for higher purity of sorted cells gated on several fluorescent and scatter properties. The downside of traditional droplet-based sorters is their operational complexity, accessibility, and potential stress on cells due to their high-pressure pumps. The WOLF (R) Cell Sorter, and WOLF G2 (R), developed by NanoCellect Biomedical, are novel microfluidic-based cell sorters that use gentle sorting technology compatible with several RNA-sequencing platforms. The experiments highlighted here demonstrate how microfluidic sorting can be successfully used to remove debris and unwanted cells prior to genomic sample preparation resulting in more data per cell and improved library complexity. (C) 2022 Author(s).

Automated summary

Single-cell RNA-Sequencing has enabled the detection of rare cell populations, microbial populations, and cancer mutations. The quality of single-cell transcriptomics relies on effective sample preparation and cell sorting techniques that preserve RNA quality and remove dead cells or debris. The WOLF(R) Cell Sorter and WOLF G2(R) are microfluidic-based cell sorters that offer gentle sorting technology compatible with various RNA-sequencing platforms, improving data per cell and library complexity.