Journal
OPEN LIFE SCIENCES
Volume 17, Issue 1, Pages 907-916Publisher
DE GRUYTER POLAND SP Z O O
DOI: 10.1515/biol-2022-0103
Keywords
glioma; BTBD10; proliferation; apoptosis; cyclin D1; Akt
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Funding
- National Natural Science Foundation of China [30973076]
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This study found that BTBD10 is downregulated in human glioma tissue and its expression negatively correlates with the pathological grade of the tumor. BTBD10 overexpression inhibits cell proliferation, induces G0/G1 arrest, and promotes cell apoptosis.
The aim of this study was to investigate the role of BTBD10 in glioma tumorigenesis. The mRNA and protein levels of BTBD10 in 52 glioma tissues and eight normal brain tissues were determined using reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis, respectively. U251 human glioblastoma cells were infected with BTBD10-expressing or control lentiviruses. Cell growth was evaluated using the methyl thiazolyl tetrazolium (MTT) assay. Cell apoptosis and cell cycle distribution were analyzed using flow cytometry. Cyclin D1 and p-Akt levels were determined using western blot analysis. The results showed that BTBD10 mRNA and protein levels were significantly lower in glioma tissues than in normal brain tissues. Additionally, BTBD10 levels were significantly lower in high-grade gliomas than in low-grade tumors. Compared with control cells, U251 cells overexpressing BTBD10 exhibited decreased cell proliferation, increased cell accumulation at the G0/G1 phase, increased cell apoptosis, and decreased levels of cyclin D1 and p-Akt. These findings show that BTBD10 is downregulated in human glioma tissue and that BTBD10 expression negatively correlates with the pathological grade of the tumor. Furthermore, BTBD10 overexpression inhibits proliferation, induces G0/G1 arrest, and promotes apoptosis in human glioblastoma cells by downregulating cyclin D1- and Akt-dependent signaling pathways.
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