Journal
PEDIATRIC SURGERY INTERNATIONAL
Volume 38, Issue 11, Pages 1555-1567Publisher
SPRINGER
DOI: 10.1007/s00383-022-05199-8
Keywords
Hirschsprung disease; Oxidative stress; Pyroptosis; NLRP3; Caspase-1
Categories
Funding
- National Natural Science Foundation of China [81801496, 81900460, 81700449]
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This study found that oxidative stress may induce enteric nerve cell death by activating caspase-1-dependent pyroptosis through NLRP3 inflammasomes, contributing to abnormal development of the enteric nervous system.
Purpose This study determined whether oxidative stress causes the developmental abnormalities of the enteric nervous system during the embryonic period. Methods Using the test results of tissue specimens of children with Hirschsprung disease (HSCR), we established a pregnant rat model of oxidative stress and a cellular oxidative stress model to conduct related molecular, cellular, and histopathological experiments for exploration and validation. Results The results of the quantitative real-time polymerase chain reaction assay indicated overexpression of pyroptosis markers (NLRP3, ASC, and caspase-1) in HSCR lesions and newborn pups in the oxidative stress group (treated with d-galactose). The expression of cathepsin D was significantly decreased in intestinal tissues of newborn pups in the oxidative stress group compared to the control group. Reactive oxygen species scavengers (N-acetyl-cysteine, NAC), the caspase-1 inhibitor (VX-765), and the NLRP3 siRNA could reverse the release of LDH, decrease the number of propidium iodide stained cells, and reduce the percentage of TUNEL/caspase-3 double-positive cells in the H2O2-treated group. Conclusion Oxidative stress can induce the death of enteric nerve cells by activating caspase-1-dependent pyroptosis through NLRP3 inflammasomes, which may contribute to abnormal enteric nervous system development.
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