Journal
BIOENGINEERED
Volume 8, Issue 4, Pages 325-330Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2016.1241925
Keywords
cell-free; cfps; freeze dry; lyophilize; onconase; protein expression; protein synthesis; tx-tl
Categories
Funding
- National Science Foundation CBET Division CAREER Award [1254148]
- National Science Foundation Graduate Research Fellowship Program [1247046]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1254148] Funding Source: National Science Foundation
- Division Of Graduate Education
- Direct For Education and Human Resources [1247046] Funding Source: National Science Foundation
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Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.
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