Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 24, Issue 36, Pages 21588-21592Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2cp03314c
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This study demonstrates the utility of carbon-deuterium labels for selective analysis of metal sites and reveals substantial strengthening of the key Cu-Cys89 bond in plastocyanin upon association with cytochrome f.
Proteins tune the reactivity of metal sites; less understood is the impact of association with a redox partner. We demonstrate the utility of carbon-deuterium labels for selective analysis of delicate metal sites. Introduced into plastocyanin, they reveal substantial strengthening of the key Cu-Cys89 bond upon association with cytochrome f.
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