Protocol for genome-wide CRISPR knockout screens of bacterial cytotoxins in HeLa cells

Article Biochemistry & Molecular Biology

TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile

Jianhua Luo et al.

Summary: The study identified tissue factor pathway inhibitor (TFPI) as the receptor for TcdB4, a dominant virulence factor of hypervirulent clade 2 Clostridioides difficile. TFPI is highly expressed in the intestinal glands and protects the colonic epithelium from TcdB2/4. These findings reveal new mechanisms for CDI pathogenesis.

CELL (2022)

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Article Microbiology

Functional analyses of epidemic Clostridioides difficile toxin B variants reveal their divergence in utilizing receptors and inducing pathology

Zhenrui Pan et al.

Summary: Clostridioides difficile toxin B (TcdB) has four dominant variants (TcdB1-4) with diverse receptor preferences, interacting with CSPG4 and Frizzled proteins. Through mutagenesis studies, it was found that TcdB interacts with CSPG4 across multiple domains. In a mouse model, the TcdB variants induced distinguishable pathological phenotypes, suggesting different disease progression in C. difficile strains.

PLOS PATHOGENS (2021)

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Letter Cell Biology