Comprehensive metabolome analysis of intracellular metabolites in cultured cells

Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types.

Automated summary

Capillary electrophoresis mass spectrometry (CE-MS) and liquid chromatography mass spectrometry (LC-MS) are two commonly used methods for metabolite analysis, but require independent sample preparation. This article presents a sample preparation protocol for breast cancer cell lines.