Journal
STAR PROTOCOLS
Volume 3, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2022.101478
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Funding
- National Institute of Mental Health [R01 MH115012, K99 MH119319]
- National Institute of Mental Health Convergent Neuroscience Consortium [U01 MH115745]
- Stanford Brain Organogenesis Program in the Wu Tsai Neuroscience Institute
- Kwan Funds
- Senkut Research Fund
- Stanford Maternal & Child Health Research Institute (MCHRI) Postdoctoral Fellowship
- American Epilepsy Society Postdoctoral Research Fellowship
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The article presents protocols for monitoring the migration of GABAergic interneurons and network activity in forebrain assembloids. Through high-resolution imaging and analysis of neuronal migration, as well as calcium imaging of network activity, the functional characteristics of forebrain assembloids can be understood.
Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids.For complete details on the use and execution of this protocol, please refer to Birey et al. (2022).
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