Preservation effect of Lippia citriodora and Laurus nobilis nanoemulsions incorporated with polylactic acid composite film for rainbow trout fillet packaging

The present study was conducted to characterize poly (lactic acid) ???Lippia citriodora and Laurus nobilis essential oils (EOs) and Nanoemulsion-based films. Following Gas chromatography???mass spectrometry and in vitro antimicrobial activity test, 12% of Nanoemulsions of both herbs were selected for further evaluations. Accordingly, Atomic Force Microscopy and Dynamic light scattering analysis showed that the scale sizes of Nanoemulsions of L. citriodora and L. nobilis were 24-60 and 15-28 nm, respectively with no significant changes in their PDI up to 90 d. The inhibition zone of both herbs against E. coli and Staphylococcus aureus alongside MIC and MBC tests showed higher efficiency in their nanoemulsions. In conclusion, two types of incorporated PLA/L. citriodora films could effectively extend the shelf life of rainbow trout fillet at refrigerated temperatures.

Automated summary

This study characterized poly (lactic acid) (PLA) and Lippia citriodora and Laurus nobilis essential oils (EOs) and their nanoemulsion-based films. The nanoemulsions showed excellent antimicrobial activity and were able to effectively extend the shelf life of rainbow trout fillet.