Journal
ANALYST
Volume 147, Issue 21, Pages 4768-4776Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2an01341j
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Funding
- Young Taishan Scholars Program of Shandong Province [tsqn201909124]
- Project of 20 items of University of Jinan [2019GXRC018]
- China Postdoctoral Science Foundation [2022M711319]
- Natural Science Foundation of Shandong Province China [ZR2021MH037]
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In this study, a sensitive sandwiched electrochemiluminescence (ECL) immunosensor was developed for the detection of cardiac troponin I (cTnI). The sensor showed a wide linear range and low detection limit, making it suitable for the sensitive detection of biomarkers for acute myocardial infarction.
Cardiac troponin I (cTnI) is one of the structural subunits of cardiac troponin complexes and a significant biomarker of acute myocardial infarction (AMI). Therefore, a sensitive detection of cTnI in the early stages is of great significance. Here, a sensitive sandwiched electrochemiluminescence (ECL) immunosensor was built for the detection of cTnI, where molybdenum disulfide@cuprous oxide-silver nanoparticles (MoS2@Cu2O-Ag) immobilized cTnI the capture antibody (Ab(1)), and cerium-doped zinc oxide@nitrogen-doped graphene quantum dots (Ce:ZnO@NGQDs) loaded the signal antibody (Ab(2)). The MoS2@Cu2O-Ag nanoparticles not only indicated excellent electroconductivity and biocompatibility but also provided a large specific surface. Ce:ZnO as a co-reaction accelerator can convert the Ce4+ <-> Ce3+ reaction and might increase the rate of electron exchange to accomplish signal enhancement. Under optimum conditions, the sensor possessed a wide linear range from 10 pg mL(-1) to 100 ng mL(-1), and the LOD was 2.90 fg mL(-1) (S/N = 3). In addition, the sensor plays a good role in serum detection, indicating that it has potential for application in the detection of biomolecules.
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