Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 2, Issue 3, Pages 368-374Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.5b00477
Keywords
H5 hemagglutinin; influenza; nanoparticle; PDEAEM; polyanhydride; hydrogel; combination nanovaccine
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Funding
- U.S. Army Medical Research and Materiel Command [W81XWH-09-1-0386, W81XWH-10-1-0806]
- Iowa State University Nanovaccine Initiative
- Stanley Chair
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H5N1 influenza virus has the potential to become a significant global health threat, and next generation vaccine technologies are needed. In this work, the combined efficacy of two nanoadjuvant platforms (polyanhydride nanoparticles and pentablock copolymer-based hydrogels) to induce protective immunity against H5N1 influenza virus was examined. Mice received two subcutaneous vaccinations (day 0 and 21) containing 10 mu g of H5 hemagglutinin tamer alone or in combination with the nanovaccine platforms. Nanovaccine immunization induced high neutralizing antibody titers that were sustained through 70 days postimmunization. Finally, mice were intranasally challenged with A/H5N1 VNH5N1-PR8CDC-RG virus and monitored for 14 days. Animals receiving the combination nanovaccine had lower viral loads in the lung and weight loss after challenge in comparison to animals vaccinated with each platform alone. These data demonstrate the synergy between polyanhydride nanoparticles and pentablock copolymer-based hydrogels as adjuvants in the design of a more efficacious influenza vaccine.
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