Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 2, Issue 10, Pages 1752-1762Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00149
Keywords
biofabrication; hydrogels; visible light; cell encapsulation; gelatin
Categories
Funding
- Royal Society of New Zealand Rutherford Discovery Fellowship [RDF-UOO1204]
- Health Research Council of New Zealand [HRC15/483]
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Oxygen inhibition is a phenomenon that directly impacts the print fidelity of 3D biofabricated and photopolymerized hydrogel constructs. It typically results in the undesirable physical collapse of fabricated constructs due to impaired cross-linking, and is an issue that generally remains unreported in the literature. In this study, we describe a systematic approach to minimizing oxygen inhibition in photopolymerized gelatin-methacryloyl (Gel-MA)-based hydrogel constructs, by comparing a new visible-light initiating system, Vis + ruthenium (Ru)/sodium persulfate (SPS) to more conventionally adopted ultraviolet (UV) + lrgacure 2959 system. For both systems, increasing photoinitiator concentration and light irradiation intensity successfully reduced oxygen inhibition. However, the UV + 12959 system was detrimental to cells at both high 12959 concentrations and UV light irradiation intensities. The Vis + Ru/SPS system yielded better cell cytocompatibility, where encapsulated cells remained >85% viable even at high Ru/SPS concentrations and visible-light irradiation intensities for up to 21 days, further highlighting the potential of this system to biofabricate cell-laden constructs with high shape fidelity, cell viability, and metabolic activity.
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