Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 2, Issue 12, Pages 2276-2286Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00492
Keywords
proteoliposomes; malaria; merozoite surface protein; GPI anchors; antitoxic response
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Funding
- FAPESP [2012/23306-5, 2015/17174-7, 2012/18617-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/23306-5, 12/18617-1] Funding Source: FAPESP
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Liposomes loaded with GPI-anchored proteins induce a strong and effective response against protozoan pathogens when immunized in mice. On the basis of results using Plasmodium falciparum merozoite proteins loaded on liposomes, we tested whether a similar approach would be protective in the P. yoelii XL and P. berghei NK65 models of lethal murine malaria infections. We also analyzed the enhancing capacity of additional adjuvants. As expected, merozoite protein loaded liposomes provided strong humoral responses (IgG titers of 10(5) against MSPl(19)) in BALB/c and CS7BL/6 host backgrounds and showed total protection in lethal challenges (100% survival) with P. yoelii, while protection against lethal challenge with P. berghei NK was at least partial (11%-20% in C57BL/6 mice and no protection in BALB/c). Thus, immunization with proteoliposomes is not only highly immunogenic but also provides a protective response in widely used murine models of malaria.
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