Journal
3 BIOTECH
Volume 6, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-016-0512-9
Keywords
Saccharomyces cerevisiae; Cellulase; Sestc gene; Engineering strain; Ethanol; In situ fermentation
Categories
Funding
- Anhui Natural Science Foundation [1408085MC67]
- Anhui Key Technology Research and Development Program [1604a0702001]
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Bioethanol is an important oil substitute produced by the sugar fermentation process. To improve the efficiency of cellulase expression of Saccharomyces cerevisiae, a eukaryotic expression vector harboring a single-enzyme-system-three-cellulase gene (sestc) was integrated into the S. cerevisiae genome by the protoplast method. Using PCR screening, RT-PCR, and transparent circle'' detection, several recombinant S. cerevisiae strains, capable of efficiently expressing the heterogeneous cellulase, were selected. The total activity of cellulase, endo-beta-D-glucanase, exo-beta-D-glucanase, and xylanase of the recombinant S. cerevisiae transformant (designated number 14) was 1.1, 378, 1.44, and 164 U ml(-1), respectively, which was 27.5-, 63-, 24-, and 19-fold higher than that of the wild-type strain. The concentration of ethanol produced by the engineered S. cerevisiae strain was 8.1 gl(-1), with wheat bran as the carbon source, under submerged conditions; this was 57.86-fold higher than that produced by the wild-type strain (0.14 gl(-1)).
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