Journal
STEM CELL REPORTS
Volume 7, Issue 5, Pages 854-868Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2016.09.008
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Funding
- National Basic Research Program of China, 973 Program of China [2012CB966503, 2015CB964902, 2015CB964901]
- National Natural Science Foundation of China [31500948, 81200337]
- Cooperation Grant of Natural Science Foundation of Guangdong Province [2014A030312012]
- Science and Information Technology of Guangzhou Key Project [201508020258]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015293, 2014327]
- Frontier and key technology innovation special grant from the Department of Science and Technology of Guangdong province [2014B020225005, 2014B020225006, 2016B030229008]
- International Science & Technology Cooperation Program of China [2014DFA30180]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01020202]
- Hundred Talents Program'' of the Chinese Academy of Science
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The transition from hemogenic endothelial cells (HECs) to hematopoietic stem/progenitor cells (HS/PCs), or endothelial to hematopoietic transition (EHT), is a critical step during hematopoiesis. However, little is known about the molecular determinants of HECs due to the challenge in defining HECs. We report here the generation of GATA2(w/eGFP) reporter in human embryonic stem cells (hESCs) to mark cells expressing GATA2, a critical gene for EHT. We show that during differentiation, functional HECs are almost exclusively GATA2/eGFP(+). We then constructed a regulatory network for HEC determination and also identified a panel of positive or negative surface markers for discriminating HECs from non-hemogenic ECs. Among them, ITGB3 (CD61) precisely labeled HECs both in hESC differentiation and embryonic day 10 mouse embryos. These results not only identify a reliable marker for defining HECs, but also establish a robust platform for dissecting hematopoiesis in vitro, which might lead to the generation of HSCs in vitro.
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