Journal
STEM CELL REPORTS
Volume 7, Issue 1, Pages 126-137Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2016.06.004
Keywords
-
Categories
Funding
- Sir Henry Dale Fellowship
- UK Regenerative Medicine Platform
- MRC [MR/K026666/1] Funding Source: UKRI
- Medical Research Council [MR/K026666/1] Funding Source: researchfish
Ask authors/readers for more resources
The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available