Evaluation and comparison of antioxidant abilities of five bioactive molecules with C-H and O-H bonds in thermodynamics and kinetics

In this work, the antioxidant abilities of NADH coenzyme analogue BNAH, F420 reduction prototype analogue F420H, vitamin C analogue iAscH(-), caffeic acid, and (+)-catechin in acetonitrile in chemical reactions were studied and discussed. Three physical parameters of the antioxidant XH, homolytic bond dissociation free energy Delta G degrees(XH), self-exchange HAT reaction activation free energy Delta G(XH/X)(not equal), and thermo-kinetic parameter Delta Gn(not equal)degrees(XH), were used to evaluate the antioxidant ability of XH in thermodynamics, kinetics, and thermo-kinetics. By comparing Delta G degrees(XH), Delta G(XH/X)(not equal) and Delta G(not equal)degrees(XH) of these five bioactive antioxidants to release hydrogen atoms, it is easy to find that iAscH(-) is the best hydrogen atom donor both thermodynamically and kinetically among these antioxidants. Caffeic acid is the worst hydrogen atom donor thermodynamically, and F420H is the worst hydrogen atom donor kinetically. In addition, the thermodynamic hydride donating abilities of BNAH, F420H, and iAscH(-) were also discussed, and the order of thermodynamic hydride donating abilities was BNAH > F420H > iAscH(-). Four HAT reactions BNAH/DPPH center dot, (+)-catechin/DPPH center dot, F420H/DPPH center dot, and caffeic acid/DPPH center dot in acetonitrile at 298 K were studied by the stopped-flow method. The actual order of H-donating abilities of these four antioxidants in the HAT reactions is consistent with the order predicted by thermo-kinetic parameters. It is feasible to predict accurately the antioxidant abilities of antioxidants using thermo-kinetic parameters.

Automated summary

This study investigates and compares the antioxidant abilities of five bioactive antioxidants in chemical reactions, finding that iAscH(-) is the best hydrogen atom donor both thermodynamically and kinetically. It demonstrates the feasibility of accurately predicting the antioxidant abilities of antioxidants using thermo-kinetic parameters.