4.6 Article

Copper(II) complexes with 2-ethylpyridine and related hydroxyl pyridine derivatives: structural, spectroscopic, magnetic and anticancer in vitro studies

Journal

RSC ADVANCES
Volume 12, Issue 42, Pages 27648-27665

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ra05133h

Keywords

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Funding

  1. Polish Ministry of Science and Higher Education
  2. National Science Centre, Poland [2021/05/X/ST4/00312A]
  3. Polish National Science Centre [2016/23/D/ST5/00269, 2020/37/N/ST4/02698]

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The properties and reaction mechanisms of different copper complexes were studied through synthesis and characterization, and their toxicity against various cancer cell lines was tested. The use of micelles to load the complexes improved their solubility and reduced side effects.
Copper(II) complexes with 2-ethylpyridine (1 and 2), 2-(hydroxyethyl)pyridine (3) and 2-(hydroxymethyl)pyridine (4) have been synthesized and characterized. All inorganic compounds have been studied by X-ray diffraction, thermogravimetry, vibrational and EPR spectroscopy as well as theoretical methods. The geometry of the complexes 1, 3 and 4 adopts nearly perfect geometry close to square planar (1, 4) or square pyramid (3) stereochemistry, respectively. The distortion of five coordinated copper(II) ions in complex 2 indicates intermediate geometry between square pyramidal and trigonal pyramidal geometry. Further, the magnetic measurements have shown antiferromagnetic behaviour of the prepared complexes in a wide range of temperatures. The antiferromagnetic behaviour of 2 should originate from the superexchange interactions between each copper(II) ion by the mixed chloride and mu 4-O ion pathways. Besides, the weak antiferromagnetic character of 2 can be also attributed to the presence of intrachain exchange between dimeric units through double oxide ion. In complex 3, strong antiferromagnetic coupling between Cu(II) centres in the Cu2O2Cl2 moiety is found. The cytotoxicity of all compounds was tested in vitro against various cancer cell lines: human lung adenocarcinoma (A549), human breast adenocarcinoma (MCF7), human prostate carcinoma; derived from metastatic site: brain (DU-145) and two normal cell lines: human embryonic kidney (HEK293T) and human keratinocyte (HaCat). Furthermore, Pluronic P-123 micelles loaded with selected complexes (1 and 3) were proposed to overcome low solubility and to minimize systemic side effects. More detailed study revealed that complex 3 loaded inside micelles causes DU-145 cells' death with simultaneous decrease of mitochondrial membrane potential and a high level of reactive oxygen species generation. The stability of the compounds 1-4 in DMSO was confirmed by UV-Vis and FT-IR spectra studies.

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