4.6 Article

On the intrinsic sterility of 3D printing

Journal

PEERJ
Volume 4, Issue -, Pages -

Publisher

PEERJ INC
DOI: 10.7717/peerj.2661

Keywords

3D printing; Cell culture; Microbiology; Sterile technique; Methods; PLA; Polylactic acid; Pasteurization

Funding

  1. Alfred P. Sloan Foundation
  2. AT&T Research Labs Fellowship
  3. BEACON Center for the Study of Evolution in Action
  4. University of Michigan Rackham Merit Fellowship
  5. Molecular Mechanisms of Microbial Pathogenesis training program [NIH T32 AI007528]

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3D printers that build objects using extruded thermoplastic are quickly becoming commonplace tools in laboratories. We demonstrate that with appropriate handling, these devices are capable of producing sterile components from a non-sterile feedstock of thermoplastic without any treatment after fabrication. The fabrication process itself results in sterilization of the material. The resulting 3D printed components are suitable for a wide variety of applications, including experiments with bacteria and cell culture.

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