Journal
PEERJ
Volume 4, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.1919
Keywords
Desulfovibrio; Rnf; Proton motive force; ionophore; Energetics
Categories
Funding
- Physical Biosciences program, DOE Office of Basic Energy Sciences, Chemical Sciences, Geosciences and Biosciences Division
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Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 mu M than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (Delta psi), Delta pH and proton motive force were lower in the rnfA mutant than in the parental strain of D.alaskensis G20. These results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.
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