Journal
PEERJ
Volume 4, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.2005
Keywords
Sorghum; Gene cloning; qRT-PCR; CCR; Phylogenetic analysis
Categories
Funding
- young talent project of Anhui province [gxyqZD2016217]
- key project of natural science research of Anhui provincial education department [KJ2016A177]
- National Natural Science Foundation of China [31301383]
- Key-construction Subject Plan of Anhui Province [WanJiaoMiKe[2014]28]
- University outstanding youth talent support program [WanJiaoMiRen[2015]211]
- youth talent support program of Anhui Science and Technology University [XiaoRenFa[2015]69]
Ask authors/readers for more resources
Cinnamoyl-CoA reductase (CCR) is the first enzyme in the monolignol-specific branch of the lignin biosynthetic pathway. In this research, three sorghum CCR genes including SbCCR1, SbCCR2-1 and SbCCR2-2 were cloned and characterized. Analyses of the structure and phylogeny of the three CCR genes showed evolutionary conservation of the functional domains and divergence of function. Transient expression assays in Nicotiana bentharniana leaves demonstrated that the three CCR proteins were localized in the cytoplasm. The expression analysis showed that the three CCR genes were induced by drought. But in 48 h, the expression levels of SbCCRI and SbCCR2-2 did not differ between CK and the drought treatment; while the expression level of SbCCR2-1 in the drought treatment was higher than in CK. The expression of the SbCCR1 and SbCCR2-1 genes was not induced by sorghum aphid [Melartaphis sacchari (Zehntner) attack, but SbCCR2-2 was significantly induced by sorghum aphid attack. It is suggested that SbCCR2-2 is involved in the process of pest defense. Absolute quantitative real-time PCR revealed that the three CCR genes were mainly expressed in lignin deposition organs. The gene copy number of SbCCRI was significantly higher than those of SbCCR2-1 and SbCCR2-2 in the tested tissues, especially in stem. The results provide new, insight into the functions of the three CCR genes in sorghum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available