Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway

Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-D-Arg-containing autum-nalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications.

Automated summary

The prenyl-D-Arg-containing autum-nalamide A is identified as a member of the cyanobactin family, and the AutF prenyltransferase can target the guanidinium moiety in arginine and homoarginine, making it a useful tool for biotechnological applications.