High Light Intensity from Blue-Red LEDs Enhance Photosynthetic Performance, Plant Growth, and Optical Properties of Red Lettuce in Controlled Environment

Plant factories using artificial light to produce vegetables have high energy costs due to the high demand for electricity for lighting. Compared to conventional light sources, light-emitting diodes (LEDs) offer the possibility of tailoring the light spectrum and regulating light intensity and are more energy-efficient in terms of energy conversion regardless of the levels of lighting intensity. Optimal light intensity and daily light integral (DLI) requirements are key factors for plant growth; however, their values vary among species and varieties. Our experiment aimed to identify the best light intensity to produce lettuce plants in controlled environment. Lettuce plants of the type Batavia cv `Blackhawk' were grown in plastic pots filled with perlite and peat (20:80 v/v) for 33 days in a growth chamber under blue (B, 20%) and red (R, 80%) LED light at a photosynthetic flux density of 130 mu mol m(-2) s(-1) (BR 130, DLI 7.49 mol m 2 d 1), 259 mu mol m(-2) s(-1) (BR 259, DLI 14.92 mol m(-2) d(-1)), and 389 mu mol m(-2) s(-1) (BR 389, DLI 22.41 mol m(-2) d(-1)). Our results showed that increasing light intensity and DLI promotes net photosynthesis, sustains the electron transport rate (ETR), and stimulates the synthesis of anthocyanins and carotenoids, with positive results for plant photoprotection. Furthermore, the decreases in vegetation indexes (photochemical reflectance index (PRI), greenness, and modified chlorophyll absorption in reflectance index (MCARI1)) also indicate changes in photosynthetic pigment content in response to plant acclimation to different DLIs. Among the three light intensities, 389 mu mol m(-2) s(-1) (DLI 22.41 mol m(-2) d(-1)) gave the best results for growing Batavia red lettuce cv `Blackhawk', since it enhances both production and qualitative traits. These results highlight the importance of a proper light intensity to promote plant growth and qualitative traits and to reach high production targets. Hence, preliminary screening of plant performance under different light treatments is recommended to optimise plant response to artificial lighting.

Automated summary

Plant factories using LED light for vegetable production can achieve higher energy conversion efficiency. The appropriate light intensity and daily light integral (DLI) are crucial for plant growth and quality. Increasing light intensity and DLI can promote net photosynthesis, stimulate pigment synthesis, and enhance plant photoprotection. Among the tested light intensities, 389 μmol m-2 s-1 showed the best results for lettuce plants, improving both yield and quality.