Journal
ANALYST
Volume 147, Issue 22, Pages 4971-4979Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2an01051h
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Funding
- Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials from MEXT, Japan
- Tokyo Tech World Research Hub Initiative (WRHI) Program of the Institute of Innovative Research, Tokyo Institute of Technology
- Nakatani Foundation, Japan
- JSPS KAKENHI from the Japan Society for the Promotion of Science, Japan [JP18H03851, JP21K14468, JP21K05268]
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This study developed a homogeneous immunosensor based on a human anti-N protein antibody for SARS-CoV-2. The addition of a crowding agent significantly improved the response speed and sensitivity of the immunosensor. The developed immunosensor was validated in testing specimens from COVID-19-positive patients.
Antigen tests for SARS-CoV-2 are widely used by the public during the ongoing COVID-19 pandemic, which demonstrates the societal impact of homogeneous immunosensor-related technologies. In this study, we used the PM Q-probe and Quenchbody technologies to develop a SARS-CoV-2 nucleocapsid protein (N protein) homogeneous immunosensor based on a human anti-N protein antibody. For the first time, we uncovered the crowding agent's role in improving the performance of the double-labeled Quenchbody, and the possible mechanisms behind this improvement are discussed. The 5% polyethylene glycol 6000 significantly improved both the response speed and sensitivity of SARS-CoV-2 Quenchbodies. The calculated limit of detection for recombinant N protein was 191 pM (9 ng mL(-1)) within 15 min of incubation, which was 9- to 10-fold lower than the assay without adding crowding agent. We also validated the developed immunosensor in a point-of-care test by measuring specimens from COVID-19-positive patients using a compact tube fluorometer. In brief, this work shows the feasibility of Quenchbody homogeneous immunosensors as rapid and cost-efficient tools for the diagnosis and high-throughput analysis of swab samples in large-scale monitoring and epidemiological studies of COVID-19 or other emerging infectious diseases.
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