Journal
THERANOSTICS
Volume 12, Issue 15, Pages 6548-6575Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.74305
Keywords
Key words; Small extracellular vesicles; exosomes; isolation techniques; clinical applications
Categories
Funding
- National Natural Science Youth Foundation of China [82001975]
- Natural Science Youth Foundation of the Jiangsu Province [BK20210074, SS2018003]
- Innovation Fund on Medicine and Education Connection of Jiangsu University [JDY2022017]
- Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation [SS2018003]
- Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and Transformation [BK20190841]
- Priority Academic Program Development of Jiangsu Higher Education Institutions Project
Ask authors/readers for more resources
Extracellular vesicles, especially small extracellular vesicles (sEVs), are important messengers in cell-to-cell communication and promising drug delivery platforms. However, the heterogeneity of their physicochemical properties and functions is not fully understood, limiting their clinical applications. This review provides up-to-date research on sEVs isolation methods, discusses their advantages and limitations, and reviews their applications in disease diagnosis and treatment.
Extracellular vesicles, especially small extracellular vesicles (sEVs) are now accepted as important messengers in cell-to-cell communication and as a promising drug delivery platform. They are involved in nearly all physiological and pathological processes and are involved in disease diagnosis and therapy. However, their heterogeneity of physicochemical properties and functions is not fully understood, which hinders further clinical applications. To obtain highly bioactive sEVs with both high yield and purity, will certainly facilitate their future study and application. This review informs up-to-date research on frequently-used and cutting-edge technologies of sEVs isolation and makes a deep comparison and analysis of different methods, including their advantages, limitations and applications. Pending questions about the inherent property of these small vesicles as well as isolation strategies are discussed. Additionally, an overview of their applications in disease diagnosis and treatment, including some of the on-going clinical trials, are also reviewed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available