4.1 Article

Spectroscopic investigation and chemical fingerprint of Datura innoxia dry biomass

Journal

COMPTES RENDUS CHIMIE
Volume 25, Issue -, Pages 227-235

Publisher

ACAD SCIENCES
DOI: 10.5802/crchim.169

Keywords

FTIR-ATR; Fluorescence spectroscopy; Secondary metabolites; Tropane alkaloids; Hyoscine; Soxhlet method

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This study applies spectroscopic methods to identify and detect secondary metabolites in Datura innoxia. The results confirm the presence of important secondary metabolite classes, such as alkaloids, terpenes, phenolics, sterols, and carbohydrates, which are useful in medicine. The study also found the presence of hyoscine in all structural units.
In this study it is presented the application of spectroscopic methods, such as fluorescence study and attenuated total reflection Fourier transform infrared analysis for identification and detec-tion of secondary metabolite specially, hyoscine, and organic functional groups from dry biomass of Datura innoxia vegetative organs. The experimental results confirmed the presence of some impor-tant classes of secondary metabolites useful in medicine domain, e.g., alkaloids, terpenes, phenolics, sterols, carbohydrates. Regarding fluorescence study, maximum excitation wavelengths for hyoscine were found to be 345 nm (1-butanol and ethanol). Moreover, the results showed the presence of hyoscine in all structural units which is in according with other studies.

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