Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 27, Issue 7, Pages 653-664Publisher
SPRINGER
DOI: 10.1007/s00775-022-01959-y
Keywords
Palladium complexes; Kinetic reactivity; DNA and BSA interactions; Cytotoxicity
Funding
- National Research Foundation (NRF-South Africa) [CPRR-98938]
- Liverpool John Moores University, UK
- University of KwaZulu-Natal
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This study synthesized a series of palladium complexes and investigated their chemical behavior and reactivity. The results showed that these complexes were stable in solution and could bind to DNA and proteins. However, they exhibited relatively low cytotoxicity against two cancer cell lines.
The pincer complexes, [Pd(L-1)Cl]BF4 (PdL1), [Pd(L-2)Cl]BF4 (PdL2), [Pd(L-3)Cl]BF4 (PdL3), [Pd(L-4)Cl]BF4 (PdL4) were prepared by reacting the corresponding ligands, 2,6-bis[(1H-pyrazol-1-yl)methyl]pyridine (L-1), bis[2-(1H-pyrazol-1-yl)ethyl]amine (L-2), bis[2-(1H-pyrazol-1-yl)ethyl]ether (L-3), and bis[2-(1H-prazol-1-yl)ethyl]sulphide (L-4) with [PdCl2(NCMe)](2) in the presence NaBF4. The solid-state structures of complexes PdL1-PdL4 confirmed a tridentate coordination mode, with one chloro ligand completing the coordination sphere to afford square-planar complexes. Chemical behaviour of the complexes in solution confirms their stability in both aqueous and DMSO stock media. The electrochemical properties of the compounds showed irreversible two-electron reduction process. Kinetic reactivity of Pd complexes with the biological nucleophiles viz, thiourea (Tu), L-methionine (L-Met) and guanosine 5 '-diphosphate disodium salt (5'-GMP) followed the order: PdL2 < PdL3 < PdL4, and PdL2 < PdL1. The kinetic reactivity is subject to the electronic effects of the spectator ligand(s), and the trend was supported by the DFT computed results. The palladium complexes PdL1-PdL4 bind to calf thymus (CT-DNA) via intercalation mode. In addition, the bovine serum albumin (BSA) showed good binding affinity to the complexes. The mode of quenching mechanism of the intrinsic fluorescence of CT-DNA and BSA by the complexes was found to be static. The order of interactions of the complexes with DNA and BSA was in tandem with the rate of substitution kinetics. The complexes, however, displayed relatively low cytotoxicity (IC50 > 100 mu M) when tested against the human cervical adenocarcinoma (HeLa) cell line and the transformed human lung fibroblast cell line (MRC-5 SV2).
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