Journal
NANOMATERIALS
Volume 6, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/nano6040056
Keywords
nanodiamond; nitrogen-vacancy center; polyglycerol; beta-lactamase tag; membrane protein
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [3306, 23115004]
- Japan Science and Technology Agency under the Core Research for Evolutional Science (CREST)
- Precursory Research for Embryonic Science and Technology (PRESTO)
- Sasakawa Scientific Research Grant
- Japan Society for the Promotion of Science under the Funding Program for Next-Generation
- Grants-in-Aid for Scientific Research [26115707, 15H03120, 15H01636, 26461576, 24685028, 15H00818, 15H01212] Funding Source: KAKEN
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The impeccable photostability of fluorescent nanodiamonds (FNDs) is an ideal property for use in fluorescence imaging of proteins in living cells. However, such an application requires highly specific labeling of the target proteins with FNDs. Furthermore, the surface of unmodified FNDs tends to adsorb biomolecules nonspecifically, which hinders the reliable targeting of proteins with FNDs. Here, we combined hyperbranched polyglycerol modification of FNDs with the beta-lactamase-tag system to develop a strategy for selective imaging of the protein of interest in cells. The combination of these techniques enabled site-specific labeling of Interleukin-18 receptor alpha chain, a membrane receptor, with FNDs, which eventually enabled tracking of the diffusion trajectory of FND-labeled proteins on the membrane surface.
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