Novel β-cyclodextrin doped carbon dots for host-guest recognition-assisted sensing of isoniazid and cell imaging

In the present study, novel beta-cyclodextrin doped carbon dots (CCDs) were prepared via a simple one-pot hydrothermal method at a mild temperature (140 degrees C), using mixtures of beta-cyclodextrin and citric acid as precursors. By characterizing the chemical properties of CCDs prepared at 140 degrees C and 180 degrees C, the importance of low-temperature reaction for preservation of the specific structure of beta-CD was elucidated. The CCDs showed excellent optical properties and were stable to changes in pH, ionic strength and light irradiation. Since the fluorescence of the CCDs could be selectively quenched by isoniazid (INZ) through specific host-guest recognition effects, a convenient isoniazid fluorescence sensor was developed. Under the optimal conditions, the sensor exhibited a relatively low detection limit of 0.140 mu g mL(-1) and a wide detection range from 0.2 mu g mL(-1) to 50 mu g mL(-1) for INZ detection. Furthermore, the sensor was employed successfully for the determination of INZ in urine samples with satisfactory recovery (91.1-109.5%), displaying potential in clinical applications. Finally, low cytotoxicity of the prepared CCDs was confirmed using the CCK-8 method, followed by application in HepG2 cell imaging.

Automated summary

In this study, novel beta-cyclodextrin doped carbon dots (CCDs) were successfully prepared, exhibiting excellent optical properties, stability, and selective recognition effects. A portable isoniazid fluorescence sensor was developed with low detection limit and wide detection range, and it showed satisfactory recovery in urine samples.