Journal
CHEMICAL SCIENCE
Volume 13, Issue 41, Pages 12149-12157Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2sc03181g
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Funding
- Natural Science Foundation of China [91753201, 21721005]
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This study presents a chemical method (m(6)A-ORL-Seq) for high-throughput detection of m(6)A modifications in the transcriptome, and successfully identifies around four thousand high-confidence m(6)A sites in the human transcriptome. Unlike traditional antibody or enzyme-based methods, this new approach provides a novel perspective for single-base m(6)A detection at the transcriptome level.
Studies of chemical modifications on RNA have ushered in the field of epitranscriptomics. N-6-Methyladenosine (m(6)A) is the most typical RNA modification and is indispensable for basic biological processes. This study presents a chemical pulldown method (m(6)A-ORL-Seq) for transcriptome-wide profiling of m(6)A. Moreover, chemical labeling results in a specific reverse transcription (RT) truncation signature. This study has identified four thousand high-confidence m(6)A sites at single-base resolution in the human transcriptome. Unlike previously reported methods based on m(6)A-antibody or m(6)A-sensitive enzymes, the antibody/enzyme-free chemical method provides a new perspective for single-base m(6)A detection at the transcriptome level.
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