Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 109, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/53633
Keywords
Bioengineering; Issue 109; Cell elimination; 3D culture; mixed cell cultures; tissue engineering; phthalocyanine; antibody-dye conjugates; photoimmunotherapy; near infrared light
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Funding
- Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research
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Recent developments in tissue engineering offer innovative solutions for many diseases. For example, tissue engineering using induced pluripotent stem cell (iPS) emerged as a new method in regenerative medicine. Although this tissue regeneration is promising, contamination with unwanted cells during tissue cultures is a major concern. Moreover, there is a safety concern regarding tumorigenicity after transplantation. Therefore, there is an urgent need for eliminating specific cells without damaging other cells that need to be protected, especially in established tissue. Here, we present a method for specific cell elimination from a mixed 3D cell culture in vitro with near infrared photoimmunotherapy (NIR-PIT) without damaging non-targeted cells. This technique enables the elimination of specific cells from mixed cell cultures or tissues.
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