Journal
CELL REPORTS MEDICINE
Volume 3, Issue 6, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.xcrm.2022.100657
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Funding
- Swedish Childhood Cancer Foundation
- Swedish Cancer Society
- Radiumhemmet Research Foundations
- Swedish Foundation for Strategic Research
- Knut and Alice Wallenberg Foundation as part of the National Bioinformatics Infrastructure Sweden at SciLifeLab
- NIH from NHLBI [R01HL131768]
- Swedish Society of Medical Research
- Gunnar-Nilsson Cancer Foundation
- National Health Services (ALF)
- Scilifelab
- National Microscopy Infrastructure, NMI [VR-RFI 2019-00217]
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Understanding the immune cell composition of human neuroblastoma is important for developing immunotherapeutics. In this study, single-cell RNA sequencing was performed on 19 neuroblastoma samples, revealing a comprehensive landscape of immune cells and identifying potential therapeutic targets and interactions.
Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. Our receptor-ligand interaction analysis reveals a highly complex interactive network of the NB microenvironment from which we highlight several interactions that we suggest for future therapeutic studies.
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