Microalgae and Cyanobacteria Biomass Pretreatment Methods: A Comparative Analysis of Chemical and Thermochemical Pretreatment Methods Aimed at Methane Production

Anaerobic digestion of microalgae and cyanobacteria was first proposed as a destination for algal biomass accumulated on stabilization ponds since it could not be disposed of directly in the environment. Now, the versatility of algal biomass makes them a suitable candidate to produce biofuels and other biomolecules in biorefineries. Anaerobic digestion of biomass is advantageous because it does not require the extraction of specific cellular constituents or drying of the biomass. Nevertheless, challenges remain regarding biomass concentration and their resistant cell walls, which are factors that could hamper methane yield. Many pretreatment methods, including chemical and thermochemical, have been proposed to break down the complex polymers present on the cell wall into smaller molecules. Unfortunately, the relationship between biomass solubilization and methane yield is not well defined. This article intends to review the anaerobic digestion of algal biomass and the role of chemical and thermochemical pretreatments in enhancing methane production. Several pretreatment conditions selected from the scientific literature were compared to verify which conditions actually improve methane yield. The severity of the selected pretreatments was also assessed using the combined severity factor. Results suggest that thermochemical pretreatment in less severe conditions is the most efficient, leading to a greater increase in methane yield. Only enzymatic pretreatments and some thermal pretreatments result in a positive energy balance. The large-scale implementation of pretreatment methods requires technological innovations to reduce energy consumption and its integration with other processes in wastewater treatment plants.

Automated summary

The anaerobic digestion of algal biomass has been proposed as a solution for the disposal and utilization of algae, which can be used to produce biofuels and other biomolecules. However, challenges remain in terms of biomass concentration and the resistant cell walls, which can affect methane yield. Various pretreatment methods have been suggested to break down complex polymers in cell walls, but the relationship between biomass solubilization and methane yield is still unclear.