Journal
ARCHIVES OF BIOLOGICAL SCIENCES
Volume 74, Issue 1, Pages 15-24Publisher
INST BIOLOSKA ISTRAZIVANJA SINISA STANKOVIC
DOI: 10.2298/ABS210929048O
Keywords
interleukin-17; periodontal ligament mesenchymal stem cells (PDLMSC); urokinase-type plasminogen activator (uPA); metalloproteinase (MMP); extracellular signal-regulated kinase (ERK)1/2; mitogen-activated protein kinase (MAPK) signaling pathway
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Funding
- Ministry of Education, Science and Technological Development, Republic of Serbia [451-03-9/2021-14/200015]
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Periodontal disease is a chronic infection that causes degradation of the periodontal tissue. Interleukin-17 (IL-17) plays a crucial role in this process by activating the ERK1/2 MAPK signaling pathway, which promotes the expression and activity of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-2 (MMP2) in periodontal ligament mesenchymal stem cells (PDLMSCs), leading to extracellular matrix degradation.
Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
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