Journal
JOURNAL OF BIOLOGICAL ENGINEERING
Volume 10, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13036-016-0024-5
Keywords
Synthetic biology; Microfluidics; DNA assembly; Transformation; Cell culture; Analysis
Funding
- Defense Advanced Research Projects Agency (DOD) [D13PC00039]
- Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy [DE-AC02-05CH11231]
- Department of Energy, ARPA-E Electrofuels Program [DE-0000206-1577]
- National Science Foundation Graduate Research Fellowship Program [DGE 1106400]
- NNF Center for Biosustainability [Synthetic Biology Tools for Yeast] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
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Background: Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. Results: Herein, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic biology research cycle: design, construction, testing, and analysis. We show the platform's capabilities for multiple automated DNA assembly methods, including a new method for Isothermal Hierarchical DNA Construction, and for Escherichia coli and Saccharomyces cerevisiae transformation. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis. Conclusions: Taken together, we demonstrate the microfluidic platform's potential to provide end-to-end solutions for synthetic biology research, from design to functional analysis.
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