Anticancer Activity and Mechanism of 2-Dodecyl-6-Ethoxycyclohexa-2,5-Diene-1,4-Dione in Liver Cancer

This study is directed to explore the activity and mechanism of 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD) against liver cancer. In vitro, we performed MTT and colony assays, AO/EB staining, flow cytometry, scratch tests, and Transwell assays. In vivo, we established a mouse model of liver cancer. HE staining, transmission electron microscopy (TEM), TUNEL assays, RT-qPCR analysis, immunohistochemical assays as well as Western blotting were employed to investigate the activity of DMDD on tumor growth. The results suggested that the proliferation and migration as well as invasion of Bel-7404 cellsIPare8.46.247.10inhibited byO:interMo,vention31withOct 2022DMDD. Fur07:41:16thermore, it promotes apoptosis and causes the cell cycle to arrest in G1 phase. DMDD can efficiently suppress the growth of liver cancer, downregulate the Copyright: American Scientific Publishers expressions of IL-2, IL-4 and IL-10 and exhibit a significant apoptosis-inducing effects of liver cancer cells in mice. More-Delivered by Ingenta over, DMDD decreases the expression of mRNA and protein of MAPK pathway. Our findings for the first time indicate that DMDD has displayed effective anticancer effects. The mechanism of the effects may be associated with the modulation of the MAPK pathway.

Automated summary

This study explores the activity and mechanism of DMDD against liver cancer, demonstrating its inhibitory effects on cell proliferation, migration, and invasion and its ability to induce apoptosis and cell cycle arrest. DMDD effectively suppresses the growth of liver cancer and downregulates the expression of IL-2, IL-4, and IL-10. It also exhibits significant apoptosis-inducing effects on liver cancer cells in mice, possibly through modulation of the MAPK pathway.