Journal
FOOD SCIENCE AND TECHNOLOGY
Volume 42, Issue -, Pages -Publisher
SOC BRASILEIRA CIENCIA TECNOLOGIA ALIMENTOS
DOI: 10.1590/fst.68222
Keywords
adsorption; heparin; kinetics; ion exchange resin
Categories
Ask authors/readers for more resources
This study investigated the recovery of heparin from porcine intestinal mucosa using an ion exchange resin. The results showed that the highest heparin yield was achieved at the lowest concentration of ammonium chloride. The pseudo-first-order model was found to be more suitable for describing the adsorption of heparin onto the resin.
The recovery of heparin from the porcine intestinal mucosa by using the ion exchange resin was studied. The procedure involved the following steps: alkaline hydrolysis, ion exchange resin adsorption, and elution. The activity of heparin was measured by an antifactor Xa assay. The ammonium chloride/ammonium hydroxide was used as a buffer, and the effect of salt concentration on the adsorption efficiency was evaluated. The results showed that the highest heparin yield (86.17%) was achieved at the lowest concentration of ammonium chloride. The pseudo-first-order model was shown as more adequate to describe heparin adsorption onto the resin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available