4.5 Article

Silencing PDCD10 Protects the Uterus Against Ischemia/Reperfusion Injury by Inhibiting Fibrosis, Inflammation and Apoptosis in Rats

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Publisher

BIOLIFE SAS
DOI: 10.23812/j.biol.regul.homeost.agents.20223603.78

Keywords

ischemia; reperfusion injury; programmed cell death 10; inflammation; fibrosis; apoptosis; uterus

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This study aimed to evaluate the role of PDCD10 in uterine ischemia/reperfusion injury (IRI) in rats. The results showed that silencing PDCD10 could ameliorate uterine IRI through inhibition of fibrosis, inflammation, and apoptosis.
Background: Programmed cell death 10 (PDCD10) is associated with cell apoptosis and inflammation, but its role in uterine ischemia/reperfusion injury (IRI) is unclear. The aim of this study was to evaluate the role of PDCD10 in uterine IRI in rats.Methods: A uterine IRI model was induced by using a microvascular clamp at the distal end of the abdominal aorta, followed by silencing smallinterfering (si) RNA-PDCD10 transfection. Histologic examinations including hematoxylin, eosin staining and Masson trichrome staining were performed. Enzyme-linked immunosorbent assays were used to determine the expression of interleukin (IL)-10, IL-6 and tumor necrosis factor (TNF)-alpha. The protein levels of PDCD10, Bcl-2, caspase-3, collagen 1, alpha- smooth muscle actin (alpha-SMA), fibroblast growth factor 2 (FGF2), IL -0, IL-6, and TNF-alpha were determined by western blot analysis.Results: The expression of PDCD10 was significantly increased in a time-dependent manner after induction of IRI, while siRNA-PDCD10 downregulated its expression. Silencing PDCD10 thickened the endometrium; thinned the myometrium; attenuated expression of collagen 1, alpha-SMA, and FGF2; repressed the expression of IL-10, IL-6, and TNF-alpha; and suppressed apoptosis by activating Bcl-2 and inhibiting Bax and caspase-3 in the uterus following IRI. Conclusions: Silencing PDCD10 ameliorated uterine IRI through inhibition of fibrosis, inflammation and apoptosis, and may serve as a promising management tool for women with uterine IRI.

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