Luminescence detection of peptide:N-glycanase activity using engineered split inteins

A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N '-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.

Automated summary

A split intein-based method has been developed to detect PNGase activity in live cells. The method utilizes protein trans-splicing between N-intein peptides and C-intein to generate an active luciferase, resulting from the cleavage of the N,N '-diacetylchitobiose linkage by PNGase.