Journal
CHEMICAL COMMUNICATIONS
Volume 58, Issue 95, Pages 13282-13285Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2cc04865e
Keywords
-
Categories
Funding
- AMED-CREST [JP22gm1410003]
Ask authors/readers for more resources
A split intein-based method has been developed to detect PNGase activity in live cells. The method utilizes protein trans-splicing between N-intein peptides and C-intein to generate an active luciferase, resulting from the cleavage of the N,N '-diacetylchitobiose linkage by PNGase.
A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N '-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available