Journal
BIOANALYSIS
Volume 14, Issue 18, Pages 1229-1239Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2022-0084
Keywords
antibody engineering; glicentin; immunoassay; oxyntomodulin; proglucagon
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This study developed a sandwich immunoassay that selectively measures endogenous oxyntomodulin (Oxm) and demonstrated its sensitivity, selectivity, and applicability for high-throughput analysis. Postprandial increases in Oxm were observed within minutes and showed significant correlation with mass spectrometry results.
Background & Aim: Oxyntomodulin (Oxm) is a proglucagon-derived peptide agonist of both the GLP-1 and glucagon receptors and is a key regulator of gastric acid secretion and energy expenditure. Differential processing from proglucagon hinders assay immunoassay selectivity. Method & results: Antibody engineering was used to develop a sandwich immunoassay that selectively measures endogenous Oxm. The pre- and postprandial levels of Oxm from 19 healthy individuals over the course of 2 h were measured. Postprandial increases in Oxm occurred within minutes and levels significantly correlated with those obtained using previously published mass spectrometry assays. Conclusion: This sandwich immunoassay is appropriately sensitive and selective and is also amenable to high-throughput application for the reliable determination of endogenous levels of intact Oxm from human samples.
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