Journal
FEBS OPEN BIO
Volume 6, Issue 12, Pages 1186-1200Publisher
WILEY
DOI: 10.1002/2211-5463.12127
Keywords
northern blot hybridization; Saccharomyces cerevisiae; small RNAs; tRNA; tRNA-derived fragments
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Funding
- Foundation for Polish Science [POMOST/2011-4/1]
- National Science Centre, Poland [2014/13/D/NZ1/00061]
- Polish Ministry of Science and Higher Education, under the KNOW program
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Short RNAs derived from the cleavage of tRNA molecules are observed in most organisms. Their occurrence seems to be induced by stress conditions, but still little is known about their biogenesis and functions. We find that the recovery of tRNA fragments depends on the RNA isolation method. Using an optimized RNA extraction protocol and northern blot hybridization technique, we show that the tRNA-derived fragments in yeast are widespread in 12 different growth conditions. We did not observe significant stress-dependent changes in the amounts of tRNA fragments pool. Instead, we show the differential processing of almost all individual tRNAs. We also provide evidence that 30-part-derived tRNA fragments are as abundant as the 50-one in Saccharomyces cerevisiae. The resulting set of S. cerevisiae tRNA fragments provides a robust basis for further experimental studies on biological functions of tRFs.
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