Journal
ANALYTICAL METHODS
Volume 14, Issue 48, Pages 5027-5033Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ay01727j
Keywords
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Funding
- National Natural Science Foundation of China
- [82030107]
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In this study, a fluorescent probe based on a self-immolation mechanism was designed to detect intracellular ONOO-. It was found that p-Borate exhibited favorable selectivity and high sensitivity to ONOO- compared to other probes, and it was successfully applied to detect ONOO- in cells and inflamed mice.
Abnormal intracellular peroxynitrite (ONOO-) concentration is related to oxidative damage, which is correlated with many pathological consequences, such as local inflammation and other diseases. In this work, a series of resorufin benzyl ether-based fluorescent probes were designed using boronate as a recognizing moiety installed on a phenyl moiety for ONOO- detection via a self-immolation mechanism. The location of the boronate as well as the substitution patterns on the phenyl moiety were investigated and the responding behaviors of the designed probes to ONOO-, other reactive oxygen species, and biothiols were examined. It was found that all the immolative probes were inevitably dominated by ONOO-. Compared with other probes, p-Borate possessed favorable selectivity and high sensitivity to ONOO-. Moreover, p-Borate was successfully used to detect ONOO- in cells and inflamed mice.
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