Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 185, Issue -, Pages 42-46Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2015.03.007
Keywords
Sea lamprey; Methylation; Metamorphosis; Epigenetics; MSAP
Categories
Funding
- Xunta de Galicia
- Fondos FEDER: Unha maneira de facer Europa [2010/80]
- Fondos FEDER: Unha maneira de facer Europa (Grupos con Potencial Crecimiento) [GPC2013-011]
- Universidade de Vigo [64102C124]
- Plan I2C fellowship from the Xunta de Galicia, Spain
- Juan de la Cierva fellowship from the Ministerio de Economia y Competitividad, Spain [CI-2011-10896]
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Lampreys represent one of the most ancient vertebrate lineages enclosing a special interest for genetic and epigenetic studies. The sea lamprey (Petromyzon marinus) is an anadromous species that experiences metamorphosis all the way up to the adult stage. Although representing a gradual process, metamorphosis in this species involves dramatic conversions with regard to physiological together with structural body changes preparing individuals for a marine and parasitic life; in consequence, multiple gene expression modifications are expected. The implications of thyroid hormones and HOX gene expression changes have previously been reported in this species and also in other vertebrate species. Nonetheless, information lacks on how these genes are regulated in lampreys. We here report about the existence of methylation pattern differences between the adult and the larvae sea lamprey life cycle stages making use of the Methylation-Sensitive Amplified Polymorphism (MSAP) technique. Differentially methylated fragment sequencing allowed to establish homologous identities with HOX genes involved in morphogenesis, along with genes related to the water balance and to the osmotic homoeostasis, all associated to a marine environment adaptation. These results provide evidences revealing that DNA methylation plays a role in the epigenetic regulation of the P. marinus post-natal development representing a starting point for future studies. To the best of our knowledge, this is the first study which detects DNA methylation changes associated with metamorphosis in lampreys. (C) 2015 Elsevier Inc. All rights reserved.
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