Journal
CLINICAL LABORATORY
Volume 68, Issue 9, Pages 1977-1981Publisher
CLIN LAB PUBL
DOI: 10.7754/Clin.Lab.2022.211243
Keywords
immunohematology; patient blood management; RBC antigens and antibodies
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This study provides a DARAEx (R) protocol for application with the Grifols platform, allowing the use of a more widespread platform in daratumumab-treated patients.
Background: The anti-CD38 antibody daratumumab is a common multiple myeloma treatment. As the erythrocyte's membrane expresses CD38, Daratumumab-treated samples show agglutination in serological pre-transfusion tests, hindering detection of erythrocyte alloantibodies. Dithiothreitol interferes with erythrocyte antigens, affecting investigation of unexpected antibodies. DARAEx (R), an anti-CD38 neutralizing agent, overcomes daratumumab-induced effects, without dithiothreitol's interferences. DARAEx (R) is applied only in Biorad columns. This study aimed to provide a DARAEx (R) protocol for application with the Grifols platform. Methods: We introduced a modified DARAEx (R) protocol (AssutaBB protocol) and performed antibody screenings on samples from nineteen daratumumab-treated patients. Results: The AssutaBB protocol provided antibody screen results for all patients, exactly as established in the default manufacturing protocol. Eleven patients presented natural negative antibody screens; eight presented positive K/E antibodies. Conclusions: AssutaBB allows the use of the more widespread Grifols platform in daratumumab-treated patients.
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