4.5 Article

Grifolamine A, a novel bis-γ-butyrolactone from Grifola frondosa exerted inhibitory effect on α-glucosidase and their binding interaction: Affinity and molecular dynamics simulation

Journal

CURRENT RESEARCH IN FOOD SCIENCE
Volume 5, Issue -, Pages 2045-2052

Publisher

ELSEVIER
DOI: 10.1016/j.crfs.2022.10.026

Keywords

Grifolamine A; Bis-?-butyrolactone; ?-glucosidase; Grifola frondosa; Absolute configuration; Surface plasmon resonance; Molecular dynamics simulation

Funding

  1. National Natural Science Foundation of China [81803393, 31901696]
  2. Guangdong Basic and Applied Basic Research Foundation [2020A1515011337]
  3. Guangdong Province Science and Technology Project [2019A050520003]
  4. GDAS Project of Science and Technology Development [2022GDASZH-2022010101]
  5. Guangdong Province Agriculture Research Project & Agricultural Technique Promotion Project [2022KJ103]
  6. Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0780]

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In this study, a novel bis-gamma-butyrolactone compound named grifolamine A (1) was isolated from the byproduct of Grifola frondosa polysaccharides preparation process. The structure of grifolamine A (1) and its inhibitory mechanism against alpha-glucosidase were determined through spectroscopic analysis and computational calculations. The results revealed a strong interaction between grifolamine A and alpha-glucosidase, providing valuable information for the design and development of novel alpha-glucosidase inhibitors based on the gamma-butyrolactone skeleton.
A novel bis-gamma-butyrolactone grifolamine A (1), the first gamma-butyrolactone dimer from nature, together with three known gamma-butyrolactones (2-4), was isolated from the byproduct from Grifola frondosa polysaccharides prepa-ration process. The structure and stereochemistry of grifolamine A (1) were elucidated by extensive spectro-scopic analysis combined with quantum chemical calculation. The biosynthetic origin of compound 1, as well as 2-4 was proposed. Grifolamine A (1) showed an intense inhibition against alpha-glucosidase in vitro. The underlying inhibitory mechanism was revealed by surface plasmon resonance (SPR), molecular docking, molecular dy-namics (MD) simulation and binding free energy calculation. SPR revealed that grifolamine A exhibited a strong affinity to alpha-glucosidase with an equilibrium dissociation constant (KD) value of 1.178 x 10-4 M. Molecular docking manifested that grifolamine A sat at the active pocket of alpha-glucosidase by van der Waals force, alkyl interaction and carbon hydrogen bonds, and consequently changed the micro-environmental structure of alpha-glucosidase. MD simulation revealed that grifolamine A had high binding affinity to alpha-glucosidase with average free energy of-25.2 +/- 3.2 kcal/mol. Free energy decomposition indicated amino acid residues including PHE298, PHE308, PHE309, PHE155 and ARG310 at the binding pocket played a strongly positive effect on the interaction between grifolamine A and alpha-glucosidase. Our findings provide valuable information for the design and development of novel alpha-glucosidase inhibitors based on gamma-butyrolactone skeleton.

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