Journal
BIOMIMETICS
Volume 7, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/biomimetics7040155
Keywords
grayscale; PDMS stamp; flexographic photopolymer mold; contact printing; 3D patterns
Funding
- National Scientific and Technical Research Council
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCYT) [PIP 2021-2023 11220200101707CO]
- Fondo para la Investigacion Cientifica y Tecnologica (FONCYT)
- Biothera Foundation
- National Institute on Minority Health and Health Disparities of the National Institutes of Health
- Florida International University Research Center in Minority Institutions [800013656]
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Microcontact printing using grayscale patterns is proposed and demonstrated in this work, which allows for the generation of different microprint pattern structures by adjusting the layout and grayscale of the stamp patterns. The functionality of this method is validated by generating specific micropatterns for complex cell differentiation. The new technique of microfabrication provides a promising alternative to conventional lithography for constructing complex aligned surfaces.
Microcontact printing using PDMS embossing tools and its variations have aroused the interest of a wide spectrum of research fields, hence the feasibility of defining micro and nanoscale patterns. In this work, we have proposed and demonstrated a novel lithography method based on grayscale patterns printed in a flexographic photopolymer mold and transferred to epoxy resin and a single PDMS stamp to obtain different microprint pattern structures. The geometry of the patterns can be modified by adjusting the layout and grayscale of the stamp patterns. The functionality of this contact printing methodology was validated by generating human induced pluripotent stem cells (hiPSC) patterns. These specific micropatterns can be very useful for achieving complex differentiation in cell lines such as hiPSC. Microfabrication through the new technique provides a promising alternative to conventional lithography for constructing complex aligned surfaces; these structures could be used as components of biological patterns or microfluidic devices.
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