Journal
NATURE PLANTS
Volume -, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41477-022-01304-w
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Funding
- JSPS KAKENHI [17H06476]
- National Science Foundation [RESEARCH-PGR 17488843]
- Howard Hughes Medical Institute
- Johnson & Johnson Centennial Chair of Plant Cell Biology at UT Austin
- Institute of Advanced Research, Nagoya University
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This study presents an atlas of dynamic chromatin landscapes during stomatal cell-lineage progression, revealing the regulatory relationships between chromatin architecture, transcription factors, and cell-fate specification. The findings shed light on the mechanism by which specific heterotypic TF complexes recruit chromatin modifiers via key co-cis regulatory elements to facilitate cell-fate commitment.
Chromatin architecture and transcription factor (TF) binding underpin cell-fate specification during development, but their mutual regulatory relationships remain unclear. Here we report an atlas of dynamic chromatin landscapes during stomatal cell-lineage progression, in which sequential cell-state transitions are governed by lineage-specific bHLH TFs. Major reprogramming of chromatin accessibility occurs at the proliferation-to-differentiation transition. We discover novel co-cis regulatory elements (CREs) signifying the early precursor stage, BBR/BPC (GAGA) and bHLH (E-box) motifs, where master-regulatory bHLH TFs, SPEECHLESS and MUTE, consecutively bind to initiate and terminate the proliferative state, respectively. BPC TFs complex with MUTE to repress SPEECHLESS expression through a local deposition of repressive histone marks. We elucidate the mechanism by which cell-state-specific heterotypic TF complexes facilitate cell-fate commitment by recruiting chromatin modifiers via key co-CREs.
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