Journal
METABOLIC ENGINEERING COMMUNICATIONS
Volume 14, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mec.2021.e00191
Keywords
Phasin; Bioplastic; Lignocellulosic; Lignin; Rhodopseudomonas palustris; Polyhydroxybutyrate
Categories
Funding
- National Science Foundation (NSF) CAREER grant [25-1106-0039-001]
- Nebraska Center for Energy Science and Research [26-1217-0020-403, 26-1217-0020-413]
- National Science Foundation (NSF) EPSCoR Center for Root and Rhizobiome Innovation grant [25-1215-0139-025]
- NSF MCB grant [25-1215-0175-001]
- U.S. Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) [2019-67012-29632]
Ask authors/readers for more resources
Heterologous expression of phasin in Rhodopseudomonas palustris can enhance the production of polyhydroxyalkanoates and improve their thermomechanical properties and potential applications.
Rhodopseudomonas palustris CGA009 is a metabolically robust microbe that can utilize lignin breakdown products to produce polyhydroxyalkanoates (PHAs), biopolymers with the potential to replace conventional plastics. Our recent efforts suggest PHA granule formation is a limiting factor for maximum production of the bioplastic poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by R. palustris. The Phap1 phasin (phaP1) from the PHB-producing model bacterium Cupriavidus necator H16 was expressed in R. palustris with the aim of overproducing PHBV from the lignin breakdown product p-coumarate by fostering smaller and more abundant granules. Expression of phaP1 yielded PHBV production from R. palustris aerobically (0.7 g/L), which does not occur in the wild-type strain, and led to a significantly higher PHBV titer than wild-type anaerobic production (0.41 g/L). The 3HV fractions were also significantly increased under both anaerobic and aerobic conditions, which boosts thermomechanical properties and potential for application. Thus, heterologous phasin expression in R. palustris provides flexibility for industrial processing and could foster compositional changes in copolymers with better thermomechanical properties compared to PHB alone.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available