The incongruity of validating quantitative proteomics using western blots

Article Biochemistry & Molecular Biology

Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation

Andreas-David Brunner et al.

Summary: Single-cell technologies have a huge impact on biology, mainly limited to imaging and deep sequencing. This study presents a robust workflow combining miniaturized sample preparation, low flow-rate chromatography, and a novel trapped ion mobility mass spectrometer to improve sensitivity. The technology allows precise and robust quantification of proteomes and their changes in single cells, providing insights into cellular heterogeneity in health and disease.

MOLECULAR SYSTEMS BIOLOGY (2022)

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Article Plant Sciences

To the proteome and beyond: advances in single-cell omics profiling for plant systems

Natalie M. Clark et al.

Summary: Recent advances in single-cell proteomics can be applied to plants, increasing our understanding of plant development, response to stimuli, and cell-to-cell signaling.

PLANT PHYSIOLOGY (2022)

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Article Biology

Science Forum: Vision, challenges and opportunities for a Plant Cell Atlas

Suryatapa Ghosh Jha et al.

Summary: With growing populations and urgent environmental issues, the future economy will be increasingly plant-based. Reimagining plant science as a critical component across various fields is crucial. The concept of a Plant Cell Atlas (PCA) is proposed to better understand and engineer plant development, physiology, and environmental responses. A workshop was held to discuss the purpose and utility of this initiative, highlighting current knowledge gaps and technical challenges.

ELIFE (2021)

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Article Multidisciplinary Sciences