Journal
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
Volume 14, Issue 12, Pages 8552-8561Publisher
E-CENTURY PUBLISHING CORP
Keywords
Vocal fold injury; animal model; Quercetin; fibrosis
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Quercetin inhibits proliferation and fibrotic changes in vocal fold fibroblasts, and has an anti-fibrotic effect after vocal fold injuries.
Objective: To investigate the effects of Quercetin on vocal fold fibroblasts induced by TGF-131 and vocal fold injury. Methods: The effects of Quercetin on collagen type I (COL-I), collagen type III (COL-III), and fibronectin (FN) expressions in transforming growth factor-131 (TGF-131)-induced human vocal fold fibroblasts were examined by the quantitative reverse transcription-polymerase chain reaction and the enzyme-linked immunosorbent assay. The Cell Counting Kit-8 was used to assess the influence of Quercetin on cell proliferation. A rat vocal fold injury model was developed. These rats were divided into a control group, a model group, and a Quercetin group. Pathorphological observations from Hematoxylin-eosin staining, the density of fibroblasts, and hypertrophic index were compared among the three groups. Results: Compared with cells untreated with TGF-131, the mRNA and protein expression levels of COL-I, COL-II, and FN and proliferation ability in TGF-131-induced human vocal fold fibroblasts were increased (all P < 0.05). Compared with human vocal fold fibroblasts induced by TGF-131, the mRNA and protein expression levels of COL-I, COL-II, and FN and proliferation ability in TGF-131-induced human vocal fold fibroblasts following Quercetin stimulation were decreased (all P < 0.05). There were no statistical differences between human vocal fold fibroblasts treated with or without Quercetin regarding cell proliferation and COL-I, COL-II, and FN expressions. Compared with the model group, Quercetin suppressed the fibrosis of the vocal fold following injury and the protein levels of COL-I, COL-II, and FN in tissue samples (all P < 0.001). The density of fibroblasts and hypertrophic index in the vocal fold scar from the Quercetin group were lower than those in the model group (all P < 0.05). Conclusions: Quercetin inhibited TGF-131-induced fibrotic changes and proliferation in human vocal fold fibroblasts and had an antifibrotic effect in vocal fold after injuries.
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